rabbit anti igf1 polyclonal antibody  (Proteintech)

Journal: European Journal of Medicinal Chemistry Reports

Article Title: Zuogui Wan attenuates ovariectomy-induced osteoporosis via the GH/IGF1 axis and related pathways

doi: 10.1016/j.ejmcr.2025.100264

Figure Lengend Snippet: Fig. 6. Effect of ZGW on the GH/IGF1 axis in OVX rats. (A) Serum GH level. (B) Serum IGF1 level. (C) The mRNA levels of Igf1 in the liver. (D) The mRNA levels of Igf1 in the tibia. (E) The mRNA levels of Igf1r in the tibia. (F) The protein expression of IGF1 in the liver. (G) The protein expression of IGF1 in the tibia. (H) The protein expression of IGF1R in the tibia. #P < 0.05 vs. SHAM group; *P < 0.05 vs. OVX group.

Article Snippet: Subsequently, the membranes were incubated with rabbit antibodies against IGF1 (Boster, ba0939, 1:800), IGF1R (Proteintech, 20254-1-AP, 1:1000), PI3K (Proteintech, 20584-1-AP, 1:1000), P-AKT (CST, 4060S, 1:1000), AKT (CST, 4691S, 1:1000) overnight at 4 ◦C, followed by the goat anti-rabbit IgG (ZSGB-Bio, ZB2301, 1:5000).

Techniques: Expressing

Journal: European Journal of Medicinal Chemistry Reports

Article Title: Zuogui Wan attenuates ovariectomy-induced osteoporosis via the GH/IGF1 axis and related pathways

doi: 10.1016/j.ejmcr.2025.100264

Figure Lengend Snippet: Fig. 8. A schematic diagram of the molecular mechanism of ZGW attenuates ovariectomy-induced osteoporosis via the GH/IGF1 axis and related pathways.

Article Snippet: Subsequently, the membranes were incubated with rabbit antibodies against IGF1 (Boster, ba0939, 1:800), IGF1R (Proteintech, 20254-1-AP, 1:1000), PI3K (Proteintech, 20584-1-AP, 1:1000), P-AKT (CST, 4060S, 1:1000), AKT (CST, 4691S, 1:1000) overnight at 4 ◦C, followed by the goat anti-rabbit IgG (ZSGB-Bio, ZB2301, 1:5000).

Techniques:

Journal: bioRxiv

Article Title: Igf2 regulates early postnatal DPP4 + preadipocyte pool expansion

doi: 10.1101/2025.02.08.637214

Figure Lengend Snippet: ( a ) Projection of two age groups (pre-wean and adult) on isolated scWAT SVCs shown as UMAP plot. ( b ) Cell Clustering of (a) based on cell types. Violin plot of ( c ) preadipocyte markers and ( d ) Igf2 . Adult primary scWAT preadipocytes were serum starved 3h and stimulated with 10 nM IGF2 for 10 min. ( e ) Western blot of phosphorylated (P)/ total (T) AKT and ERK (n=3). Immunoprecipitation (IP) of ( f ) IGF1R (n=2) and ( g ) InsR (n=2). Immunoblotting of P-Tyrosine (TYR) was used to assess the phosphorylation of the receptors after IGF2 stimulation.

Article Snippet: Unspecific binding sites were blocked using 5% bovine serum albumin (BSA) or non-fat dried milk in TBS-T (1 X TBS containing 0.1% Tween-20) for 1h at RT, followed by incubation with primary antibodies overnight at 4°C: AKT (1:1000, Cell Signaling, 4685), phosphor-AKT Ser 473 (1:1000, Cell Signaling, 9271), ERK (1:1000, Cell Signaling, 4695), phosphor-ERK (1:1000, Cell Signaling, 4377), insulin receptor (InsR) (1:1000, Cell Signaling, 3020), IGF1 receptor (IGF1R) (1:1000, Cell Signaling, 9750), PPARγ (1:1000, Cell Signaling, 2443), B-Actin HRP (only 30 min at RT, 1:5000, Santa Cruz, Sc-47778).

Techniques: Isolation, Western Blot, Immunoprecipitation, Phospho-proteomics

Journal: bioRxiv

Article Title: Igf2 regulates early postnatal DPP4 + preadipocyte pool expansion

doi: 10.1101/2025.02.08.637214

Figure Lengend Snippet: Cultured adult scWAT preadipocytes (Pre) were differentiated with (Dif. Adip + 10nM IGF2) or without (Dif. Adip) 10 nM IGF2. ( a ) mRNA expression of Igf2 (n=5). ( b ) Western blot of InsR, IGF1R and PPARγ. B-Actin was used as loading control (n=5). ( c ) Lipid quantification by Oil red O (ORO) absorbance at 500 nm normalized to DAPI of only differentiated primary adult scWAT preadipocytes (n=5). ( d ) Western blot of phosphorylated (P) and total (T) AKT of protein isolated from cultured pre-wean scWAT preadipocytes that were serum starved for 12 h with 1µg/ml IgG or IGF2 neutralizing antibody (IGF2 Ab) (n=1). ( e ) mRNA levels of Igf2 from differentiated pre-wean scWAT primary preadipocytes that were treated with 1 µg/ml IgG (Dif. Adip + IgG Cnt) or IGF2 neutralizing antibody (Dif. Adip IGF2 Ab) (n=4). ( f ) 100 nM insulin was substituted with either 10 nM IGF2 or insulin during differentiation. mRNA levels of Igf2 and AdipQ (n=3). Data shown by mean ± SEM.

Article Snippet: Unspecific binding sites were blocked using 5% bovine serum albumin (BSA) or non-fat dried milk in TBS-T (1 X TBS containing 0.1% Tween-20) for 1h at RT, followed by incubation with primary antibodies overnight at 4°C: AKT (1:1000, Cell Signaling, 4685), phosphor-AKT Ser 473 (1:1000, Cell Signaling, 9271), ERK (1:1000, Cell Signaling, 4695), phosphor-ERK (1:1000, Cell Signaling, 4377), insulin receptor (InsR) (1:1000, Cell Signaling, 3020), IGF1 receptor (IGF1R) (1:1000, Cell Signaling, 9750), PPARγ (1:1000, Cell Signaling, 2443), B-Actin HRP (only 30 min at RT, 1:5000, Santa Cruz, Sc-47778).

Techniques: Cell Culture, Expressing, Western Blot, Control, Isolation

Journal: bioRxiv

Article Title: Igf2 regulates early postnatal DPP4 + preadipocyte pool expansion

doi: 10.1101/2025.02.08.637214

Figure Lengend Snippet: Circulating levels of ( a ) insulin (n=8 pre-wean and n=5 adult) and ( b ) IGF2 (n=6 pre-wean and n=5 adult) in pre-wean and adult mice. ( c ) Secreted IGF2 levels in cell culture supernatant of pre-wean and adult primary scWAT preadipocytes (n= 3 pre-wean and n=4 adult). Primary adult preadipocytes were supplemented with (Dif. Adip + 10 nM IGF2) or without (Dif. Adip) 10 nM IGF2 during differentiation. mRNA expression levels of ( d ) Igf2 (n=5) and ( e ) adipogenic markers (n=5). ( f ) Western blot of differentiated adipocytes for InsR, IGF1R, PPARγ. B-Actin was used as loading control (n=5). ( g ) Lipid quantification by Oil Red O (ORO) (n=5). ( h ) Immunocytochemistry of F-Actin (grey) and Lipids (green) (n=4). Primary pre-wean preadipocytes were treated with 1 µg/ml IgG (Dif. Adip + IgG Cnt) or IGF2 neutralizing antibody (Dif. Adip IGF2 Ab) during differentiation. MRNA expression levels of ( i ) Igf2 (n=4) and ( j ) adipogenic markers (n=4). Data shown by mean ± SEM.

Article Snippet: Unspecific binding sites were blocked using 5% bovine serum albumin (BSA) or non-fat dried milk in TBS-T (1 X TBS containing 0.1% Tween-20) for 1h at RT, followed by incubation with primary antibodies overnight at 4°C: AKT (1:1000, Cell Signaling, 4685), phosphor-AKT Ser 473 (1:1000, Cell Signaling, 9271), ERK (1:1000, Cell Signaling, 4695), phosphor-ERK (1:1000, Cell Signaling, 4377), insulin receptor (InsR) (1:1000, Cell Signaling, 3020), IGF1 receptor (IGF1R) (1:1000, Cell Signaling, 9750), PPARγ (1:1000, Cell Signaling, 2443), B-Actin HRP (only 30 min at RT, 1:5000, Santa Cruz, Sc-47778).

Techniques: Cell Culture, Expressing, Western Blot, Control, Immunocytochemistry